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Genomic Library Construction (For more information email us)
Custom Genomic Library Construction Service is offered in BAC, cosmid, bacteriophage or plasmid vectors. High molecular weight Pulse Field Gel Electrophoresis (PFGE) isolated DNA is digested, CIP treated and size fractionated. The size fractionated DNA is ligated to a suitable vector package and is harvested.
Bionexus can construct genomic libraries from nanogram quantities of genomic DNA or milligram quantities of the tissue. Bionexus routinely generates libraries from trace amount of genomic DNA, chromosomes, uncultured environmental microbes, or base-modified phages. Highly methylated DNA contaminated with polysaccharides, phenolic compounds, or restriction enzyme resistant DNA samples are successfully used to generate libraries.
BAC vector
- Insert size-125 kb to 200kb
- 100,000 to >200,000 clones (based on required x coverage)
- Very cost effective
- Turnaround time 6-8 weeks
Cosmid vector
- Insert size 30 kb to 40 kb
- >107 primary clones
- 90-95% recombinants
- Turnaround time 4 weeks
Bacteriophage / Plasmid vector
- Insert size 9-23kb (bacteriophage vector) and 2-10 kb (plasmid vector)
- >107 primary clones
- 90-95% recombinants
- Supplied as amplified or un-amplified library
- Turnaround time 4 weeks
Clones from all the libraries can be arrayed on nylon membranes.
The libraries will be amplified once to render stability to the clones and will be titrated and supplied in SM buffer. A complete report containing the specifications of the library and other data will be provided along with the libraries.
*Gridding available only with libraries in plasmid vectors.
Estimated Delivery Date: The Genomic Library would require 4 weeks to complete after receiving
starting materials (custom vector will require additional time). Client will be the sole owner of the
Genomic Libraries, RNA, DNA, and all sequence data generated. BIONEXUS is providing a service. The estimated time required to complete your project is noted above. The project starting date will be finally determined as soon as we receive the signed quotation and all starting materials at our facility
All communications for this Service will be held in the strictest confidence.
Contact Bionexus for a price quotation and more information today.