- DNA Mass Marker
- Quantitative Plasmid DNA LADDER
- DNA Mass Marker from 100 bp - 4000 bp, with evenly spaced bands.
- Easy to remember / recognize band sizes
- EASILY STORED AND SHIPPED: At room temperature, stable for up to 1 year (Store in dark area and not under UV light)
- MASS LADDER
- PERFECT STANDARD FOR QUANTITATION
4000 bp - 75ng
2000 bp - 200ng
1000 bp - 100ng
500 bp - 50 ng
100 bp - 20ng
1 ml of premixed DNA marker with loading dye.
A. Mix thoroughly by inverting tube 2-3 times before use.
B. Load 10ul per lane. Run in 1.5% agarose gel at 200 constant volts.
Notes:< Based on 1ng of marker per application in a volume of 10ul (including dye). DNA concentration per band is as indicated. Gel was run through 14cm long gel, in TAE buffer containing Ethidium Bromide (EtBr), and visualized at the 365nm. Loading dye consists of bromophenol blue. For research use only. Not intended for animal or human diagnostic or therapeutic use.
The DNA band quantities listed above are determined by spectrophotometer, but lot to lot variation is clearly apparent.
Residual ammonium ions are present and may inhibit kinase exchange reactions.
Both marker and dye contain 0.02% sodium azide.
DNA Amount (ng) per Band
10 ul 5 ul 2 ul 4 kb 75 37.5 15 2kb 200 100 40 1 kb 100 50 20 500 bp 50 25 10 100 bp 20 10 4
100 Applications (with loading dye)
Also available without loading dye.
Storage: Store in dark area and not under UV light . The marker is stable at room temp. Long storage at 4°C. Aliquots at -20°C